Mmp 9 Antibody Cell Signaling

Anti rabbit igg hrp linked antibody 7074 was used as secondary antibody.

Mmp 9 antibody cell signaling.

Mmp 13 is synthesized as a latent proenzyme and proteolytic removal of the inhibitory propeptide domain is required for enzyme activation. Immunoprecipitation of mmp 2 protein from u 118mg cell extracts. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lys554 of human mmp 2 protein. Background the matrix metalloproteinases mmps are a family of proteases that target many extracellular proteins including other proteases growth factors cell surface receptors and adhesion.

Mmp 9 is a matrix metalloprotease produced from immune cells including macrophages and neutrophils which plays important roles in wound healing immunity and angiogenesis. Incubate with rotation overnight at 4 c. Antibodies are purified by protein a and peptide affinity chromatography. Background the matrix metalloproteinases mmps are a family of proteases that target many extracellular proteins including other proteases growth factors cell surface receptors and adhesion.

Maximum mmp activity requires proteolytic cleavage to generate active mmps by releasing the inhibitory propeptide domain from the full length protein 5. Lane 1 is 10 input lane 2 is rabbit da1e mab igg xp isotype control 3900 and lane 3 is mmp 2 d4m2n rabbit mab. Western blot analysis was performed using mmp 2 d4m2n rabbit mab as primary antibody. 2001 curr opin cell biol 13 534 40.

In this product review i report results that the anti mouse mmp 9 antibody from cell signaling showed unsurpassed performance in detecting the protein in both western blotting and immunofluroscence microscopy. Cell signaling technology the mmp 9 13667 rabbit antibody is specific and with high affinity toward its target. Add protein a agarose 10 30 µl of 50 bead slurry. It was detected by western blot in total lysate of human primary leukemia blasts.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lys115 of human mmp 9. Microcentrifuge for 30 sec at 4 c. Add primary antibody at the appropriate dilution as recommended in the product datasheet to 200 µl cell lysate at 1 mg ml. Mmp 13 is a member of a subgroup of collagenases including mmp 1 mmp 8 and mmp 18 that play an even more important function targeting fibrillar collagen.

One mechanism of mmp regulation is transcriptional 5. Incubate with rotation for 1 3 hr at 4 c. Antibodies are purified by protein a and peptide affinity chromatography. Once synthesized mmp exists as a latent proenzyme.

Matrix metalloproteinase 9 92 kda gelatinase 92 kda type iv collagenase gelatinase b mmp9 human.